Coding

Part:BBa_K4759035:Design

Designed by: Shiyue Zhao   Group: iGEM23_Jiangnan-China   (2023-10-05)


SUMO-linker-OleP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 209
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 209
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 209
    Illegal BglII site found at 98
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 209
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 209
    Illegal AgeI site found at 334
    Illegal AgeI site found at 432
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SUMO via linker at the N terminus of OleP


Source

fusion tag

References